Related Experiment Videos
Comparison of GC and HPLC for the quantification of organic acids in coffee
Gulab N Jham1, Sergio A Fernandes, Cleverson Fernando Garcia
1Universidade Federal de Viçosa, Laboratório de Pesquisa em Produtos Naturais, Departamento de Química, Viçosa, MG 36.570-000, Brazil. gulab@mail.ufv.br
Abstract:
A GC and an HPLC method for the quantification of organic acids OAs in coffee have been compared. The GC procedure, employing trimethylsilyl derivatives, was found to be very tedious. The HPLC method, which employed an ion exchange column using a flow gradient of water containing 1% phosphoric acid and UV detection (210 nm), was found to be much simpler for the quantification of eight organic acids (oxalic, succinic, fumaric, malic, tartaric, citric, quinic and fumaric acids) in four representative coffee samples. The HPLC procedure was more convenient than that described in the literature since no pre-purification was required for quantification of the OAs.