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Pressure pulsation in solid-phase fermentation.

Xiaomin Yang¹, Tom Huang, George T Tsao

¹School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Applied Biochemistry and Biotechnology

|May 23, 2002

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Pressure pulsation effectively cools solid-phase fermentation beds, enhancing enzyme production. This method controls temperature and moisture for optimal microbial growth in large-scale applications.

Area of Science:

Biotechnology
Biochemical Engineering
Industrial Microbiology

Background:

Solid-phase fermentation (SPF) is limited in large-scale applications due to inadequate heat dissipation.
Managing heat accumulation and moisture loss is critical for efficient SPF.

Purpose of the Study:

To investigate the cooling effects of pressure pulsation on solid-phase fermentation beds.
To develop an on-line control system for temperature and moisture in large-scale SPF.
To optimize enzyme production using Aspergillus niger NRRL3.

Main Methods:

Utilized pressure pulsation to enhance cooling and evaporation in porous solid beds.
Developed computer software for on-line control of temperature and moisture.
Cultivated Aspergillus niger NRRL3 on wheat bran and corncobs in a 12-L fermentor.

Related Experiment Videos

Main Results:

Maximum temperature within the solid bed was maintained below 40°C during 100 hours of fermentation.
Medium water content was successfully controlled between 61% and 65%, optimal for cell growth.
Uniform cell distribution and heavy growth were observed on the solid substrate.

Conclusions:

Pressure pulsation is an effective method for managing heat and moisture in large-scale solid-phase fermentation.
The developed on-line control system provides a superior environment for enzyme production compared to traditional flasks.
This technology enables efficient and scalable production of enzymes via solid-phase fermentation.