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Related Experiment Videos

Method for measuring postantifungal effect in Aspergillus species.

Roxana G Vitale1, Johan W Mouton, Javier Afeltra

  • 1Department of Medical Microbiology, University Medical Center Nijmegen, The Netherlands.

Antimicrobial Agents and Chemotherapy
|May 23, 2002
PubMed
Summary

This study developed an in vitro method to measure the postantifungal effect (PAFE) in molds. Amphotericin B showed a significant PAFE in Aspergillus fumigatus, while itraconazole did not induce a measurable PAFE in any tested Aspergillus species.

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Area of Science:

  • Mycology
  • Antimicrobial Susceptibility Testing

Background:

  • The postantifungal effect (PAFE) is crucial for understanding antifungal drug efficacy.
  • Standardized methods for determining PAFE in molds are lacking.
  • Aspergillus species are significant opportunistic fungal pathogens.

Purpose of the Study:

  • To develop and validate an in vitro method for determining the postantifungal effect (PAFE) in Aspergillus species.
  • To evaluate the PAFE of amphotericin B and itraconazole against various Aspergillus isolates.
  • To establish a basis for future investigations into the clinical relevance of PAFE.

Main Methods:

  • Developed an in vitro method using National Committee for Clinical Laboratory Standards guidelines (M38-P).
  • Exposed Aspergillus conidia to varying concentrations and durations of amphotericin B and itraconazole.

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  • Quantified PAFE by comparing growth curves of drug-exposed conidia to controls using optical density measurements.

    • Main Results:

    • Amphotericin B induced a significant PAFE in Aspergillus fumigatus after 2 and 4 hours of exposure.
    • A. terreus, A. ustus, and A. nidulans exhibited significantly shorter or no measurable PAFE to amphotericin B.
    • Itraconazole did not induce a measurable PAFE in any of the tested Aspergillus isolates at any concentration or exposure time.

    Conclusions:

    • The developed in vitro method allows for the determination of PAFE in molds.
    • Amphotericin B demonstrates a notable postantifungal effect against Aspergillus fumigatus, suggesting prolonged activity.
    • Further research is needed to correlate PAFE findings with clinical outcomes and optimize antifungal dosing strategies.