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Related Experiment Videos

Human papillomavirus E2 down-regulates the human telomerase reverse transcriptase promoter.

Daeyoup Lee1, Hak-Zoo Kim, Kwi Wan Jeong

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.

The Journal of Biological Chemistry
|May 23, 2002
PubMed
Summary

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Human papillomavirus E2 protein represses human telomerase reverse transcriptase (hTERT) gene expression by targeting the Sp1 binding site. This finding offers new insights into E2

Area of Science:

  • Molecular Biology
  • Virology
  • Cancer Research

Background:

  • Transcriptional regulation of human telomerase reverse transcriptase (hTERT) is crucial for cell transformation and differentiation.
  • Human papillomavirus (HPV) E2 protein is known to inhibit cell growth and induce apoptosis in infected cells.

Purpose of the Study:

  • To investigate the mechanism by which HPV E2 protein modulates cellular gene expression, specifically focusing on its effect on the hTERT promoter.
  • To determine if HPV E2 protein influences hTERT gene expression, a key factor in cellular immortalization and cancer development.

Main Methods:

  • Reporter gene assays to map the E2-responsive region of the hTERT promoter.
  • Site-directed mutagenesis to identify critical binding sites for E2-mediated repression.

Related Experiment Videos

  • Chromatin immunoprecipitation (ChIP) assays to confirm E2 recruitment to the hTERT promoter.

    • Main Results:

    • HPV E2 protein was demonstrated to inhibit the hTERT promoter activity.
    • The Sp1 binding site on the hTERT promoter was identified as crucial for E2-mediated repression in 293T cells.
    • E2-mediated repression of the hTERT promoter was independent of E-Box binding, and E2 was actively recruited to the promoter region.

    Conclusions:

    • HPV E2 protein directly represses hTERT gene transcription through interaction with the Sp1 binding site.
    • These findings elucidate a novel mechanism of HPV E2 protein action, impacting cellular processes like senescence and potentially contributing to viral oncogenesis.
    • The study provides new insights into the biological functions of HPV E2 protein in regulating key genes involved in cell proliferation.