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[Functional analysis of SpltMNPV envelope protein SL136]
1State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|May 23, 2002
Summary
The Spodoptera litura nucleopolyhedrovirus Sl136 protein is an early gene and a budded virus envelope protein. Its cell surface localization is crucial for Spodoptera litura multinucleocapsid nucleopolyhedrovirus virulence.
Area of Science:
- Virology
- Molecular Biology
- Insect Pathology
Context:
- Spodoptera litura multinucleocapsid nucleopolyhedrovirus (SpltMNPV) is a significant insect pathogen.
- Previous research indicated the Sl136 product's membrane fusion capabilities.
- Understanding viral gene function is key to developing novel pest control strategies.
Purpose:
- To characterize the SpltMNPV Sl136 gene and its protein product.
- To determine the temporal expression pattern of Sl136.
- To elucidate the role of Sl136 in viral infection and virulence.
Summary:
- RT-PCR confirmed Sl136 as an early gene, transcribed at 6 hours post-infection.
- Antiserum confirmed SL136 as a budded virus (BV) envelope protein, with a molecular weight of approximately 65 kD.
- SL136 protein localized to the cell surface in infected cells, as shown by cell ELISA (CELISA).
- Furin cleavage of SL136 was not essential for viral propagation, but glycosylation inhibition reduced BV virulence.
Impact:
- Identifies Sl136 as a critical BV envelope protein involved in SpltMNPV infection.
- Provides insights into the post-translational modification and localization of viral proteins.
- Suggests that inhibiting Sl136 glycosylation could be a potential strategy for controlling SpltMNPV outbreaks.