Related Experiment Videos
Cancare-a herbal formulation inhibits chemically induced tumours in experimental animals
1Amala Cancer Research Centre, Thrissur, India.
Abstract:
Cancer chemopreventive potential of Cancare, a multi-herbal formulation on chemically induced tumours was studied by N-nitrosodiethylamine (NDEA) induced hepatocarcinogenesis in rats and 20-methylcholanthrene (20-MC) induced sarcoma development in mice. Oral administration of Cancare was found to inhibit the liver tumour development induced by N-nitrosodiethylamine. Animals administered with NDEA had visible liver tumours by the end of 30th weeks and the liver weight was raised to 6.1 +/- 1.4 g/ 100 g body wt. None of the animals treated with Cancare (150 mg/ kg) developed any visible liver tumours by this period and the liver weight was 3.0 +/- 0.6 g/ 100 g body wt. Gamma-Glutamyl transpeptidase, a marker of hepatocellularcarcinoma, which was raised to 83.7 +/- 8. 9 U/l in serum of NDEA treated group was reduced to 35.2 +/- 6.1 U/l by simultaneous administration of Cancare. Elevated levels of serum alkaline phosphatase, glutamate pyruvate transaminase, bilirubin, liver glutathione S-transferase, glutathione and gamma-Glutamyl transpeptidase in the NDEA administered group was significantly reduced by Cancare administration. Cancare administration inhibited the sarcoma development and increased the life span of mice administered with 20-MC dose dependently. All animals in the control group developed sarcomas by 150th day and dead by 174th day after 20-MC administration. Cancare administration (30 mg and 150 mg/kg) inhibited the sarcoma development (46.7 and 60%) as well as increased the life span (53.3 and 66.7%) as estimated on 240th day after 20-MC administration. The results are indicative of the chemopreventive potential of Cancare against chemically induced neoplasmas.
Insights
This study shows that Cancare, a multi-herbal formulation, effectively prevents chemically induced liver tumors and sarcomas in animal models. Cancare demonstrated significant inhibition of cancer development and increased lifespan, indicating its chemopreventive potential.
Area of Science:
- Pharmacology
- Oncology
- Natural Products
Background:
- Chemically induced tumors are a significant research model for cancer.
- Multi-herbal formulations are increasingly investigated for therapeutic potential.
- Hepatocarcinogenesis and sarcoma development models are crucial for evaluating anticancer agents.
Purpose of the Study:
- To evaluate the chemopreventive efficacy of Cancare, a multi-herbal formulation.
- To assess Cancare's effect on N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats.
- To determine Cancare's impact on 20-methylcholanthrene (20-MC)-induced sarcoma development in mice.
Main Methods:
- Oral administration of Cancare to rats and mice exposed to chemical carcinogens (NDEA and 20-MC).
- Monitoring of tumor development, liver weight, and serum marker levels (e.g., Gamma-Glutamyl transpeptidase).
- Assessment of sarcoma incidence and lifespan in mice treated with Cancare.
Main Results:
- Cancare significantly inhibited liver tumor development and reduced elevated liver weight in NDEA-treated rats.
- Serum markers associated with hepatocellular carcinoma were normalized by Cancare administration.
- Cancare dose-dependently inhibited sarcoma development and increased lifespan in 20-MC-treated mice.
Conclusions:
- Cancare exhibits significant chemopreventive potential against chemically induced hepatocarcinogenesis and sarcoma.
- The multi-herbal formulation demonstrates efficacy in reducing tumor burden and improving survival rates.
- These findings support further investigation of Cancare as a natural chemopreventive agent.