Separation of thiazide diuretics and antihypertensive drugs by thin-layer chromatography

A procedure is reported for the thin-layer chromatographic (TLC) separation and identification of thiazide diuretics and other antihypertensive drugs. Several new solvent systems and a variety of possible detection reagents were examined. Twenty thiazides used routinely in therapy were successfully separated and identified. Use of the TLC systems and combinations of the detecting methods described should be useful for the identification of these drugs in biological fluids and in various dosage forms.