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Vitamin E protects renal antioxidant enzymes and attenuates glomerulosclerosis in Adriamycin-treated rats
Christiane Van den Branden1, An Deman, Bart Ceyssens
1Department of Human Anatomy, Vrije Universiteit Brussel, Belgium.
Background/Aims:
In the rat Adriamycin model of chronic renal failure, the development of glomerulosclerosis and tubulointerstitial lesions is accompanied by decreased activities and mRNA levels of the antioxidant enzymes. In this study, we investigated the effect of oral vitamin E supplementation on antioxidant enzyme activities in both the cortex and isolated glomeruli from Adriamycin-treated rats.
Methods:
Glomerulosclerosis, tubulointerstitial lesions and ferric iron deposits were evaluated by histochemical staining methods, and antioxidant enzyme activities were measured by spectrophotometry.
Results:
Vitamin E supplementation of the normal diet attenuates Adriamycin-induced glomerulosclerosis and tubulointerstitial lesions, but not proteinuria and serum total cholesterol, low-density lipoprotein cholesterol, triglycerides and total protein concentrations. In the cortex, vitamin E completely prevented a decrease in enzyme activity for Cu/Zn superoxide dismutase and catalase, and partly for Mn superoxide dismutase and glutathione peroxidase. In the glomeruli, vitamin E completely prevented a decrease in activity for Cu/Zn superoxide dismutase, catalase and glutathione peroxidase, and partly for Mn superoxide dismutase.
Conclusion:
Dietary supplementation of vitamin E protects the activities of antioxidant enzymes in the kidney cortex and glomeruli, and attenuates the evolution towards terminal renal failure in rats treated with Adriamycin.
Insights
Vitamin E supplementation protected antioxidant enzyme activities in rat kidneys damaged by Adriamycin. This dietary intervention attenuated kidney lesions and slowed progression toward renal failure.
Area of Science:
- Nephrology
- Biochemistry
- Oxidative Stress Research
Background:
- Adriamycin-induced chronic renal failure in rats involves glomerulosclerosis and tubulointerstitial damage.
- This damage is associated with reduced activity and mRNA levels of key antioxidant enzymes.
- The study investigates the protective role of vitamin E in this model.
Purpose of the Study:
- To determine the effect of oral vitamin E supplementation on antioxidant enzyme activities.
- To assess vitamin E's impact on kidney lesions in the Adriamycin-induced renal failure model.
- To evaluate vitamin E's influence on specific renal compartments: cortex and glomeruli.
Main Methods:
- Utilized the rat Adriamycin model to induce chronic renal failure.
- Histochemical staining assessed glomerulosclerosis, tubulointerstitial lesions, and iron deposits.
- Spectrophotometry measured antioxidant enzyme activities (Cu/Zn SOD, Mn SOD, catalase, glutathione peroxidase).
Main Results:
- Vitamin E supplementation attenuated Adriamycin-induced glomerulosclerosis and tubulointerstitial lesions.
- Oral vitamin E preserved the activity of several antioxidant enzymes in both the renal cortex and isolated glomeruli.
- Vitamin E did not significantly affect proteinuria or serum lipid profiles.
Conclusions:
- Dietary vitamin E protects antioxidant enzyme activities within the kidney cortex and glomeruli.
- Vitamin E supplementation mitigates the progression of renal damage in Adriamycin-treated rats.
- This suggests a potential therapeutic role for vitamin E in managing certain types of chronic kidney disease.
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