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CD80 expression is induced on activated B cells following stimulation by CD86
N C Sahoo1, K V S Rao, K Natarajan
1Immunology group, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Scandinavian Journal of Immunology
|May 25, 2002
Summary
Ligation of CD86 on activated B cells upregulates CD80 expression, impacting immune responses. This CD86-induced CD80 enhances T cell costimulation and cytokine secretion.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD86 and CD80 are crucial costimulatory molecules in T cell activation.
- The regulation of CD80 expression on B cells is not fully understood.
Purpose of the Study:
- To investigate the effect of CD86 ligation on CD80 surface expression and mRNA levels in activated B cells.
- To determine if CD86-induced CD80 can function as a costimulatory molecule.
Main Methods:
- Activated splenic B cells with enhanced CD86 levels were treated with anti-CD86 monoclonal antibody (MoAb).
- Cell surface CD80 levels and CD80 mRNA were quantified.
- Coculture experiments with CD28-expressing fibroblasts and CTLA-4-Ig were performed.
- T cell costimulation assays were conducted to assess CD80 function.
Main Results:
- Anti-CD86 MoAb treatment significantly upregulated cell surface CD80 and CD80 mRNA in activated B cells.
- CD86-upregulated B cells enhanced CD80 levels upon coculture with CD28-expressing cells, but not with CTLA-4-Ig expressing cells.
- CD86-induced CD80 demonstrated costimulatory function by enhancing allogeneic T cell cytokine secretion.
Conclusions:
- CD86 ligation represents a novel pathway for regulating CD80 expression on activated B cells.
- This regulation occurs at the level of CD80 mRNA.
- CD86-induced CD80 plays a functional role in T cell costimulation, potentially influencing immune responses.