Related Experiment Video
Updated: Aug 11, 2026
08:04
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Stress-induced protein CSP 310: a third uncoupling system in plants
A V Kolesnichenko1, T P Pobezhimova, O I Grabelnych
1Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Irkutsk-33, P.O. Box 1243, Irkutsk, 664033, Russia. akol@sifibr.irk.ru
Planta
|May 25, 2002
Summary
Cold-stress protein CSP 310 enhances non-phosphorylative respiration in plant mitochondria. Its uncoupling mechanism differs from known systems, suggesting a novel pathway in cereals.
Area of Science:
- Plant Physiology
- Mitochondrial Respiration
- Cold Stress Response
Background:
- Plant mitochondria possess uncoupling proteins that regulate respiration.
- Cold stress response involves specific proteins like CSP 310.
- Understanding uncoupling mechanisms is crucial for plant adaptation.
Purpose of the Study:
- To investigate the effect of cold-stress-related protein CSP 310 on plant mitochondrial respiration.
- To elucidate the mechanism of action of CSP 310 in isolated plant mitochondria.
- To compare CSP 310's uncoupling activity with known plant uncoupling systems.
Main Methods:
- Isolation of mitochondria from winter wheat, winter rye, maize, and pea.
- Addition of CSP 310 to isolated mitochondria and measurement of non-phosphorylative respiration.
- Inhibition studies using KCN and assessment of fatty acid dependence.
Main Results:
- CSP 310 addition increased non-phosphorylative respiration in all tested plant mitochondria.
- The increase in respiration was KCN-sensitive, ruling out alternative oxidase activity.
- CSP 310's uncoupling effect was independent of free fatty acids, unlike PUMP.
Conclusions:
- CSP 310 acts as an uncoupler of mitochondrial respiration in plants.
- The mechanism of CSP 310 uncoupling is distinct from known plant uncoupling proteins.
- CSP 310 represents a novel, third uncoupling system identified in cereal mitochondria.
Related Concept Videos
01:27C4 Pathway and CAM
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
02:04Regulation of Transpiration by Stomata
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
01:57Adaptations that Reduce Water Loss
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
02:45Responses to Heat and Cold Stress
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
01:46The Calvin Benson Cycle
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
01:24Protein Transport to the Stroma
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...