Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in 4-aminobiphenyl-induced methemoglobinemia in Cyp1a2(-/-) knockout mice.

Howard G Shertzer1, Timothy P Dalton, Glenn Talaska

  • 1Department of Environmental Health, Center for Environment Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267-0056, USA. shertzhg@ucmail.uc.edu

Toxicology and Applied Pharmacology
|May 29, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Replicating lipid micelles: a feasible precursor to the origin of life and the earliest appearance of genomes.

Human genomics·2026
Same author

Update of the Methyltransferase Gene Family: Classification, Evolution and Biological Functions.

Human genomics·2026
Same author

Lessons learned from aldehyde dehydrogenases as non-P450 aldehyde-oxidizing enzymes: implications for the 'exposome' and human health.

Expert opinion on drug metabolism & toxicology·2025
Same author

Update of the sideroflexin (SLC56) gene family.

Human genomics·2025
Same author

Preliminary human health risk assessment of antibiotic exposures in human waste handling occupations.

Journal of occupational and environmental hygiene·2024
Same author

Species differences in specific ligand-binding affinity and activation of AHR: The biological basis for calculation of relative effective potencies and toxic equivalence factors.

Regulatory toxicology and pharmacology : RTP·2024
Same journal

Anti-metastatic effects of emodin-8-O-β-D-glucopyranoside in breast cancer: Targeting EMT, MMPs, and angiogenesis.

Toxicology and applied pharmacology·2026
Same journal

Corrigendum to 'Human-relevant exposure to mixtures of rare earth elements disrupts T3 homeostasis through impaired synthesis and conversion in human Thyrocytes' [Toxicology and Applied Pharmacology volume 514 (2026) 117929].

Toxicology and applied pharmacology·2026
Same journal

Per- and polyfluoroalkyl substances and the risk of thyroid and breast cancer: Evidence from a meta-analysis and computational toxicology.

Toxicology and applied pharmacology·2026
Same journal

High expression of Tbx15 in lung adenocarcinoma cells facilitates bone metastasis by suppressing IL-25 secretion and reducing MVP expression in osteoclasts.

Toxicology and applied pharmacology·2026
Same journal

Corrigendum to "Nitazoxanide reverses pulmonary vascular remodeling in pulmonary hypertension by targeting the IMPA1-RAGE signaling axis" [Toxicology and Applied Pharmacology 507 (2026) 117657].

Toxicology and applied pharmacology·2026
Same journal

Time-resolved transcriptomic responses delineate liver injury, immune-mediated clearance, and regeneration following microcystin-LR exposure in whitefish: implications for an uptake-limiting defense response (autokleisis).

Toxicology and applied pharmacology·2026
See all related articles

Occupational exposure to arylamines can cause methemoglobinemia. This study found that the absence of cytochrome P450 1A2 (CYP1A2) enzyme enhances this effect, suggesting individual CYP1A2 levels impact arylamine toxicity.

Area of Science:

  • Toxicology
  • Biochemistry
  • Occupational Health

Background:

  • Arylamines are occupational toxins that can cause methemoglobinemia.
  • Cytochrome P450 1A2 (CYP1A2) is implicated in the metabolic activation of arylamines.
  • Methemoglobin formation is a biomarker for arylamine exposure.

Purpose of the Study:

  • To investigate the role of CYP1A2 in 4-aminobiphenyl (ABP)-induced methemoglobinemia.
  • To compare methemoglobin levels in CYP1A2 knockout mice and wild-type mice after ABP exposure.

Main Methods:

  • Topical application of 4-aminobiphenyl (ABP) to Cyp1a2(-/-) and wild-type Cyp1a2(+/+) mice.
  • Measurement of methemoglobin levels at various time points post-exposure.
  • Assessment of hepatic thiol levels.

Related Experiment Videos

  • Treatment with dioxin to modulate CYP activity.

    • Main Results:

    • ABP exposure depleted hepatic thiols in both genotypes.
    • Methemoglobin levels were significantly higher in Cyp1a2(-/-) mice compared to wild-type mice.
    • Dioxin treatment reduced methemoglobin levels in both genotypes.

    Conclusions:

    • CYP1A2 does not play a positive role in ABP-induced methemoglobin formation.
    • The absence of CYP1A2 enhances ABP-induced methemoglobinemia.
    • Individual variations in human CYP1A2 levels may influence susceptibility to arylamine toxicity.