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Related Experiment Videos

Calbindin-D(28K) cells selectively contact intra-SCN neurons.

J LeSauter1, L J Kriegsfeld, J Hon

  • 1Department of Psychology, Barnard College, New York, NY 10027, USA.

Neuroscience
|May 29, 2002
PubMed
Summary

Calbindin-D(28K) cells in the hamster suprachiasmatic nucleus receive input from various peptides. These cells show specialized topographical organization and reciprocal connections, crucial for understanding circadian rhythm regulation.

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Area of Science:

  • Neuroscience
  • Chronobiology
  • Cell Biology

Background:

  • Calbindin-D(28K)-immunoreactive cells are located in the suprachiasmatic nucleus (SCN) of hamsters.
  • These cells receive direct retinal input and are activated by light pulses, indicated by Fos expression.
  • Understanding the afferent and efferent connections of these cells is vital for comprehending SCN organization.

Purpose of the Study:

  • To investigate the interconnections between calbindin-D(28K) cells and other peptidergic cells within the SCN.
  • To map the afferent and efferent connections of calbindin-D(28K) cells in the hamster SCN.

Main Methods:

  • Epi- and confocal microscopy were employed to visualize cellular interactions.
  • Intra-suprachiasmatic nucleus tract tracing techniques were utilized to identify neuronal projections.

Related Experiment Videos

  • Biotinylated dextran amine injections into the SCN were used to trace efferent pathways.

    • Main Results:

    • Calbindin-D(28K) cells receive significant input from vasoactive intestinal polypeptide (VIP), neuropeptide Y, and serotonin fibers.
    • Most calbindin-D(28K) cells also receive input from gastrin-releasing peptide (GRP) and cholecystokinin (CCK) fibers.
    • Reciprocal connections were observed with VIP, GRP, and CCK cells, but not with dorsomedial vasopressin cells.

    Conclusions:

    • The study reveals a highly specialized topographical organization of connections within the SCN.
    • A significant percentage of substance P, GRP, and VIP cells within the calbindin subnucleus coexpress calbindin-D(28K).
    • These findings enhance our understanding of neuronal circuitry and information processing in the SCN, the master circadian clock.