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Endogenous biotin staining in a subset of spinal neuronal cell bodies: a potential confounding factor for
1Department of Zoology, 730 Van Vleet Oval, University of Oklahoma, Norman, OK 73019, USA. ari@ou.edu
Brain Research
|May 29, 2002
Abstract:
Biotinylated compounds are commonly used to label neuronal cell bodies via intracellular filling or retrograde tracing. Endogenous concentrations of biotin within a subset of neuronal cell bodies would pose a problem for interpreting such experiments. Here I report that a subset of turtle spinal cord neuronal cell bodies strongly stains for biotin, using the avidin-biotin-horseradish peroxidase (ABC) reaction, in the absence of any exogenous biotinylated compound.