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Nitric oxide and hydroxyl radicals initiate lipid peroxidation by NMDA receptor activation
Yoshihito Hayashi1, Yuto Ueda, Akira Nakajima
1Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889-1692, Japan.
Brain Research
|May 29, 2002
Abstract:
In this experiment, we used direct electron paramagnetic resonance (EPR) spectra to measure lipid peroxidation by hydroxyl radical (.OH), nitric oxide (.NO) and lipid radical (.L). NMDA-receptor associated lipid peroxidation is thought to act through .OH in induction of neurotoxicity. The origin of .OH generation was found to arise mainly from peroxynitrite anion produced from O(2)(-) and .NO rather than from Fenton's reaction. This study verified that .OH generation from interactive reactions between .NO and O(2)(-) initiates NMDA-induced lipid peroxidation of PC12 cells.