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The structure of the alpha-keratin microfibril
1Division of Protein Chemistry, CSIRO, Parkville, Victoria, Australia.
Bioscience Reports
|June 1, 1983
Summary
X-ray diffraction reveals alpha-keratin microfibrils have a periodic surface lattice structure. This finding refines models of intermediate filament frameworks, explaining recent observations.
Area of Science:
- Structural biology
- Biophysics
- Materials science
Background:
- Alpha-keratin is a key component of intermediate filaments.
- Previous models of alpha-keratin microfibrils exist but require refinement.
- Understanding intermediate filament structure is crucial for cell biology.
Purpose of the Study:
- To quantitatively analyze X-ray diffraction patterns of alpha-keratin.
- To refine existing models of alpha-keratin microfibril structure.
- To provide a framework for understanding other intermediate filaments.
Main Methods:
- Quantitative analysis of X-ray diffraction patterns.
- Comparison of experimental data with theoretical models.
- Integration of chemical and structural evidence.
Main Results:
- X-ray diffraction data are consistent with a microfibril surface lattice.
- A periodic interruption with an axially projected period of 470 A was identified.
- The findings support an elaborated model for the alpha-helical framework.
Conclusions:
- The proposed microfibril structure explains physicochemical and electron-microscopical observations.
- This model offers a basis for developing frameworks for other intermediate filaments.
- Further research can build upon this refined structural model.