Related Experiment Videos
Phospholipid and protein analysis of pulmonary surfactant
R C Cottrell1, I A Herod, D Bayley
1The British Industrial Biological Research Association, Carshalton, Surrey, UK.
Bioscience Reports
|June 1, 1983
Abstract:
Quantitative analysis of the phospholipid composition of pulmonary surfactant preparations has been achieved by a modification of the phosphorus-31 nuclear magnetic resonance method of E London and G Feigenson (J. Lipid Res. 20, 408-412, 1979). Resolution of the protein components by a 2-dimensional isoelectric-focussing-SDS/polyacrylamide-gel-electrophoresis technique is reported for the first time.