Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide and peroxynitrite interactions with mitochondria.

Rafael Radi1, Adriana Cassina, Roberto Hodara

  • 1Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. rradi@fmed.edu.uy

Biological Chemistry
|May 30, 2002
PubMed
Summary

Nitric oxide (NO) and peroxynitrite (ONOO-) impact mitochondria, affecting respiration, dysfunction, and cell death. Understanding these interactions is key to mitochondrial health and disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental factors modulate the responsiveness and reversibility of the H<sub>2</sub>O<sub>2</sub>-induced inactivation of GAPDH.

Free radical biology & medicine·2026
Same author

Aortic Stenosis Progression: Lessons from Multimodality Imaging.

Mayo Clinic proceedings·2026
Same author

Evolution of allostery without shape shifting: Internal dynamics drives functional diversification of a transcriptional repressor superfamily.

bioRxiv : the preprint server for biology·2026
Same author

Monoclonal antibodies against nitrated nerve growth factor reveal an oxidation-dependent pathogenic hallmark in ALS.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hepatic metabolites indicate differences during late mid-lactation in Holstein cows with different levels of pasture inclusion.

Scientific reports·2026
Same author

Multifaceted roles for persulfide species in redox chemical biology.

Nature chemical biology·2026

Area of Science:

  • Mitochondrial biochemistry
  • Cellular signaling
  • Reactive nitrogen species metabolism

Background:

  • Nitric oxide (NO) and peroxynitrite (ONOO-) are reactive nitrogen species with significant biological roles.
  • Mitochondria are central players in cellular energy production and apoptosis.
  • Dysregulation of NO and ONOO- signaling is implicated in various pathologies.

Purpose of the Study:

  • To review the biochemical mechanisms by which NO and ONOO- interact with mitochondrial components.
  • To elucidate the consequences of these interactions on mitochondrial function and cellular fate.
  • To highlight the role of mitochondria as targets for reactive nitrogen species.

Main Methods:

  • Literature review of biochemical and cellular studies.

Related Experiment Videos

  • Analysis of signaling pathways involving NO and ONOO- in mitochondria.
  • Examination of oxidative damage to mitochondrial components.

    • Main Results:

    • Physiological NO levels reversibly inhibit cytochrome c oxidase, altering mitochondrial membrane potential and calcium uptake.
    • High NO levels induce mitochondrial oxidant formation.
    • Peroxynitrite causes oxidative damage to key mitochondrial proteins, including electron transport chain complexes and enzymes.
    • Mitochondrial scavenging systems partially mitigate ONOO- toxicity.
    • Nitrated mitochondrial proteins are detected in vivo, confirming mitochondria as targets.

    Conclusions:

    • Mitochondria are critical sites for NO and ONOO- action, influencing respiration, dysfunction, and apoptosis.
    • The balance of NO and ONOO- levels dictates their impact on mitochondrial health.
    • Understanding these mechanisms is crucial for developing therapeutic strategies against diseases involving reactive nitrogen species.