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Current developments in LC-MS for pharmaceutical analysis.

Chang-Kee Lim1, Gwyn Lord

  • 1MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck, University of London, UK. c.lim@sbc.bbk.ac.uk

Biological & Pharmaceutical Bulletin
|May 30, 2002
PubMed
Summary

This review covers liquid chromatography-mass spectrometry (LC-MS) advancements for pharmaceutical analysis. It details ionization techniques, chromatographic interfaces, and applications in drug metabolism, impurity profiling, and accelerated drug discovery.

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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Science

Background:

  • Liquid chromatography-mass spectrometry (LC-MS) is a powerful analytical technique.
  • Its application in pharmaceutical analysis is rapidly evolving.

Purpose of the Study:

  • To review current developments in LC-MS.
  • To discuss its applications in pharmaceutical analysis.

Main Methods:

  • Description of various mass spectrometric ionization techniques (e.g., electrospray, nanospray, atmospheric pressure chemical ionization, photoionization).
  • Discussion of interfaces with liquid chromatographic techniques (e.g., high-performance liquid chromatography, capillary electrophoresis, capillary electrochromatography).
  • Evaluation of the advantages and disadvantages of each technique.

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Main Results:

  • LC-MS is crucial for in vitro and in vivo drug metabolism studies.
  • It enables identification and characterization of pharmaceutical impurities.
  • Analysis of chiral impurities in drug substances is facilitated by LC-MS.
  • High-throughput LC-MS-MS systems accelerate drug discovery.

Conclusions:

  • LC-MS is a versatile and indispensable tool in modern pharmaceutical analysis.
  • Advancements in LC-MS continue to drive innovation in drug discovery and quality control.