[Determination of the time of polypeptide chain synthesis in animal experiments]

A method is worked out for the estimation of the time of polypeptide chain synthesis in animals in vivo. The time of the synthesis of "middle" polypeptide was found to be 1.45 min. in mouse liver cells under normal conditions, while in the presence of translation inhibitors, cycloheximide (20 mg/kg) and aurintricarboxilic acid (1 g/mg) the time of the synthesis increased in 2.7 and 2.5 times respectively. The time of polypeptide synthesis linearly increased with the increase of aurintricarboxilic acid dose.