Producing a highly immunogenic synthetic vaccine construct active against HIV-1 subtype C

A synthetic peptide immunogen designated the multiple epitope immunogen (MEI), based on the principle neutralizing domain (PND) of the third variable region (V3) of the HIV-1 subtype C surface envelope protein was prepared by combining two different modes of synthesis [Vaccine 12 (8) (1994) 736; AIDS Res. Hum. Retroviruses 14 (9) (1998) 751; J. Biol. Chem. 263 (4) (1988) 1719]. The MEI induced strong humoral immune responses (titers of >100) in Swiss White and Balb/c mice and antibodies from individuals with HIV/AIDS recognize the immunogen at similar antibody titers. Anti-MEI antibodies were detected by ELISA (using HIV-1 and the immunogen as antigen) and confirmed by proliferation assays (stimulation indices of >5).