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5-Halogenated thymidine analogues induce a senescence-like phenomenon in HeLa cells
Eriko Michishita1, Naoto Matsumura, Toshihiro Kurahashi
1Division of Biochemistry, Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
Abstract:
We tested various thymidine analogues for induction of a senescence-like phenomenon in HeLa cells. CldU, BrdU, and IdU similarly induced the morphology of senescent cells and typical senescence markers. Thymidine analogues other than 5-halogenated forms caused only cell death. BrdU efficiently killed the cells in cooperation with irradiation with light and a brief treatment with Hoechst 33258, but CldU did not at all. 5-Halogenated thymidine analogues were thus shown to be specific inducers of cellular senescence in mammalian cells.