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Developmental expression of PAM (protein associated with MYC) in the rodent brain
Huaitao Yang1, Klaus Scholich, Steve Poser
1Department of Anatomy & Neurobiology, University of Tennessee Health Science Center, 855 Monroe Avenue, Memphis, TN 38163, USA.
Abstract:
Recently, human protein associated with MYC, PAM, has been cloned and characterized as a large protein that interacts with the transcriptional-activating domain of Myc. The regional expression pattern of PAM in brains has not been yet been defined. Expression patterns of PAM in both rat and mouse brains were examined by using in situ hybridization. Here, we demonstrate that PAM mRNA is highly expressed in specific anatomical regions including hippocampus, dentate gyrus and cerebellum. In these areas, PAM mRNA is restricted to pyramidal cells of hippocampus and granule cells of dentate gyrus and cerebellum. During development, PAM mRNA expression is differentially regulated. It is turned on after birth and up-regulated during the first postnatal 2 weeks. Thereafter, PAM mRNA expression remains elevated into adulthood. The regional distribution of PAM in brain is similar to that observed for several adenylyl cyclase isoforms such as type I isoform. However, no obvious alterations of PAM mRNA expression are detected in brains of mice deficient in type I or type 8 or type 1 and type 8 isoforms of adenylyl cyclase. Thus, adenylyl cyclase does not appear to alter the expression of PAM.
Insights
Protein associated with MYC (PAM) mRNA is highly expressed in specific brain regions like the hippocampus and cerebellum. Its expression is regulated during development and is independent of adenylyl cyclase isoforms.
Area of Science:
- Neuroscience
- Molecular Biology
- Gene Expression Analysis
Background:
- The protein associated with MYC (PAM) is a large protein interacting with Myc's transcriptional-activating domain.
- The regional expression pattern of PAM within the brain has not been previously defined.
- Understanding PAM's brain distribution is crucial for elucidating its function in neural tissues.
Purpose of the Study:
- To define the regional expression pattern of PAM mRNA in the adult and developing rat and mouse brains.
- To investigate the cellular localization of PAM mRNA in key brain structures.
- To explore the relationship between PAM expression and adenylyl cyclase isoforms.
Main Methods:
- In situ hybridization was employed to examine PAM mRNA expression patterns.
- Analysis was conducted on both rat and mouse brain tissues.
- Comparison of PAM expression was made in wild-type and adenylyl cyclase-deficient mice (types I, 8, and combined I/8).
Main Results:
- PAM mRNA exhibits high expression in the hippocampus, dentate gyrus, and cerebellum.
- Within these regions, PAM mRNA is localized to pyramidal cells (hippocampus) and granule cells (dentate gyrus, cerebellum).
- PAM mRNA expression begins postnatally, increases significantly in the first two weeks, and remains high in adulthood.
- No significant alterations in PAM mRNA expression were observed in mice lacking adenylyl cyclase types I or 8.
Conclusions:
- PAM mRNA is regionally expressed in specific neuronal populations within the rat and mouse brain.
- PAM expression is developmentally regulated, increasing after birth and stabilizing in adulthood.
- Adenylyl cyclase isoforms (type I and 8) do not appear to influence PAM mRNA expression levels in the brain.