A human gene, ATP6E1, encoding a testis-specific isoform of H(+)-ATPase subunit E
Related Experiment Video
Updated: Oct 1, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
Published on: June 20, 2018
Yoko Imai-Senga1, Ge-Hong Sun-Wada, Yoh Wada
1Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Mihogaoka 8-1, Ibaraki-shi, Osaka 567-0047, Japan.
Abstract:
We have identified a novel human gene, ATP6E, encoding an E subunit isoform of vacuolar-type proton-translocating ATPase (V-ATPase). ATP6E1 was mapped to approximately 2p16-p12 on chromosome 2, and has a simple genomic organization: a noncoding exon and a coding one for an E1 isoform separated by a 6.1 kb intron, with boundaries following the GT-AG rule. Transcription initiation sites were found at -375 and -158 bases upstream of the translation initiation codon. Northern blotting analysis demonstrated that ATP6E1 is specifically transcribed in testis as 1.1 kb and 2.2 kb mRNAs, whereas the previously reported ATP6E2 (E2) is expressed in all tissues tested. E1 exhibited 76.9% identity with ubiquitously expressed E2, and both isoforms functionally complemented null mutations of the yeast counterpart VMA4, indicating that they are bona fide subunits of the V-ATPase complex.
Related Concept Videos
ATP Synthase: Mechanism
ATP Synthase: Structure
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
ATP Driven Pumps III: V-type Pumps
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The ADP/ATP Carrier Protein

