Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p53 expression in the normal murine eye.

Russell Pokroy1, Yevgeny Tendler, Ayala Pollack

  • 1Department of Ophthalmology, Kaplan Medical Center, Rehovot, Israel.

Investigative Ophthalmology & Visual Science
|May 31, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treating late-onset Tay Sachs disease: Brain delivery with a dual trojan horse protein.

Molecular therapy. Methods & clinical development·2024
Same author

Ganglion Cell Layer Thickness Change in Neovascular Age-Related Macular Degeneration Treated With Anti-VEGF Injections.

Journal of vitreoretinal diseases·2023
Same author

CYSTOID MACULAR EDEMA AFTER FOUR-POINT SCLERAL FIXATION OF INTRAOCULAR LENS.

Retina (Philadelphia, Pa.)·2021
Same author

LASIK versus PRK for high astigmatism.

International ophthalmology·2021
Same author

CENTRAL RETINAL VEIN OCCLUSION WITH SEVERE MACULAR EDEMA IN EARLY PREGNANCY.

Retinal cases & brief reports·2020
Same author

Features of p53 protein distribution in the corneal epithelium and corneal tear film.

Scientific reports·2020

The tumor suppressor p53 protein is highly expressed in normal mouse eye tissues, particularly the cornea, conjunctiva, and lens epithelium, with distinct staining patterns observed in each. This research maps p53 expression in the murine eye.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Cell Biology

Background:

  • The p53 gene encodes a crucial phosphoprotein regulating cell growth, with known roles in malignancy and apoptosis.
  • Recent findings indicate p53 expression in normal tissues, including the eye.

Purpose of the Study:

  • To map and characterize the expression of p53 in normal murine ocular tissues.
  • To identify specific ocular tissues with significant p53 protein levels.

Main Methods:

  • Adult C57BL/6 mouse eyes were analyzed post-mortem.
  • Indirect immunofluorescence microscopy was used to map p53 expression in frozen eye sections.
  • ELISA and Western blot analyses quantified p53 expression in dissected ocular tissues.

Main Results:

Related Experiment Videos

  • Strong p53 protein expression was detected in various normal ocular tissues.
  • Corneal and conjunctival epithelium showed high cytoplasmic p53 levels.
  • Lens epithelial cells and lens fiber nuclear bow exhibited high nuclear p53 staining; retinal tissue had low levels.

Conclusions:

  • Normal murine ocular tissues, especially the cornea, conjunctiva, and lens epithelium, exhibit high p53 protein levels.
  • Distinct staining patterns of p53 were observed in these ocular tissues.
  • The study provides a detailed map of p53 expression in the healthy mouse eye.