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Related Experiment Videos

Automated ribotyping of vancomycin-resistant Enterococcus faecium isolates.

Sylvain Brisse1, Vivian Fussing, Ben Ridwan

  • 1Eijkman-Winkler Institute, University Medical Centre Utrecht, 3584 CX Utrecht, The Netherlands. sbrisse@lab.azu.nl

Journal of Clinical Microbiology
|May 31, 2002
PubMed
Summary

Automated ribotyping effectively identifies Vancomycin-resistant Enterococcus faecium (VREF) strains with high interlaboratory reproducibility. EcoRI enzyme is recommended for characterizing VREF, aiding in the detection of hospital outbreak strains.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Typing

Background:

  • Vancomycin-resistant Enterococcus faecium (VREF) poses a significant threat in hospital-acquired infections.
  • VREF strains are prevalent in both community and agricultural settings in Europe.

Purpose of the Study:

  • To evaluate automated ribotyping for interlaboratory reproducibility and discriminatory power.
  • To compare automated ribotyping with Amplification of Fragment Length Polymorphism (AFLP) and Multilocus Sequence Typing (MLST).
  • To assess the capacity of ribotyping for identifying epidemiologically important VREF strains.

Main Methods:

  • Automated ribotyping using EcoRI and BamHI restriction enzymes.
  • Comparison with AFLP analysis and MLST.
  • Interlaboratory reproducibility testing with 19 (EcoRI) and 16 (BamHI) isolates.

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  • Manual refinement of automated fingerprint analysis.

    • Main Results:

    • High interlaboratory reproducibility rates of 95% (EcoRI) and 100% (BamHI) were achieved after manual refinement.
    • Ribotyping differentiated VREF strains into 28 (EcoRI) and 25 (BamHI) types, comparable to AFLP and MLST.
    • Ribotyping identified host-specific genogroups and strains carrying the esp gene, a marker for hospital outbreaks.

    Conclusions:

    • Automated ribotyping, particularly with EcoRI, demonstrates high discriminatory power and interlaboratory reproducibility for VREF characterization.
    • This method is valuable for identifying epidemiologically significant VREF genotypes, including those associated with hospital outbreaks.
    • Automated ribotyping is a recommended approach for VREF strain characterization.