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Updated: Oct 1, 2026
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA
Heli Nummelin1, Yasmin El Tahir, Pauli Ollikka
1Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Viikinkaari 1, PO Box 65, FIN-00014 University of Helsinki, Finland.
Abstract:
A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype O:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A resolution on a synchrotron-radiation source.
