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Updated: Oct 1, 2026
A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
Published on: June 5, 2026
Crystallization and preliminary crystallographic studies on the chromo shadow domain (CSD) of mouse heterochromatin
Yu Gao1, Yi Ding, Prim B Singh
1Laboratory of Structural Biology and MOE Laboratory of Protein Science School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Abstract:
Members of the heterochromatin protein 1 (HP1) class of non-histone chromosomal proteins are components of heterochromatin and are involved in the epigenetic regulation of the genome. HP1 proteins are modular and consist of two sequence-related domains called the chromodomain (CD) and the chromo shadow domain (CSD). In order to investigate the role of the murine HP1-like protein M31 in heterochromatin formation and gene silencing, recombinant CSD was overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method with PEG 4000 as precipitant. Diffraction data to 2.9 A were collected from a native crystal belonging to space group C222(1), with unit-cell parameters a = 60.0, b = 95.6, c = 91.7 A, alpha = beta = gamma = 90 degrees.
