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Related Experiment Videos

Oxidative biotransformations using oxygenases.

Zhi Li1, Jan B van Beilen, Wouter A Duetz

  • 1Institute of Biotechnology, ETH Zurich, Hoenggerberg, CH-8093, Zurich, Switzerland. zhi@biotech.biol.ethz.ch

Current Opinion in Chemical Biology
|June 1, 2002
PubMed
Summary

Researchers have advanced oxidative biotransformations using oxygenases, improving enzyme properties and enabling large-scale synthesis of pharmaceutical intermediates. In vitro applications are now feasible with cofactor regeneration systems.

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Area of Science:

  • Biocatalysis and enzyme engineering
  • Organic synthesis
  • Pharmaceutical chemistry

Background:

  • Oxygenases are crucial enzymes for oxidative biotransformations.
  • Significant advancements have been made in enhancing oxygenase performance.
  • Biocatalysis offers a sustainable route for synthesizing complex molecules.

Purpose of the Study:

  • To summarize progress in manipulating oxygenases for biotransformations.
  • To highlight improvements in enzyme characteristics like selectivity and activity.
  • To discuss the scalability and in vitro applications of oxygenase-catalyzed reactions.

Main Methods:

  • Enzyme engineering and substrate engineering to improve oxygenase function.
  • Biocatalyst screening for optimal enzyme selection.

Related Experiment Videos

  • Whole-cell biotransformations using wild-type or recombinant strains.
  • Enzymatic or electrochemical regeneration of NADH/NADPH for in vitro systems.

    • Main Results:

    • Substantial improvements in substrate acceptance, catalytic activity, regioselectivity, and stereoselectivity of oxygenases.
    • Successful gram to several-hundred-gram scale preparative biotransformations.
    • Demonstration of in vitro synthetic applications of oxygenases through cofactor regeneration.

    Conclusions:

    • Oxygenases are powerful tools for oxidative biotransformations with enhanced properties.
    • Scalable synthesis of pharmaceutical intermediates and chiral synthons is achievable.
    • In vitro applications of oxygenases are viable, expanding their synthetic utility.