Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipases as practical biocatalysts.

Manfred T Reetz1

  • 1Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470, Mülheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de

Current Opinion in Chemical Biology
|June 1, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Learning from Protein Engineering by Deconvolution of Multi-Mutational Variants.

Angewandte Chemie (International ed. in English)·2024
Same author

Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation.

Methods in enzymology·2023
Same author

Breakthrough in the chemoenzymatic transformation of CO<sub>2</sub> for the sustainable artificial synthesis of hexoses with complete control of stereochemistry.

Science bulletin·2023
Same author

Rational enzyme design for enabling biocatalytic Baldwin cyclization and asymmetric synthesis of chiral heterocycles.

Nature communications·2022
Same author

The Unexplored Importance of Fleeting Chiral Intermediates in Enzyme-Catalyzed Reactions.

Journal of the American Chemical Society·2021
Same author

Publisher Correction: A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes.

Scientific reports·2021

This review covers recent advances in using lipases, which are key enzymes in organic synthesis. It highlights practical methods for improving lipase activity and enantioselectivity in producing chiral intermediates.

Area of Science:

  • Enzymology
  • Organic Chemistry
  • Biocatalysis

Background:

  • Lipases are versatile enzymes widely employed in synthetic organic chemistry.
  • They catalyze ester hydrolysis in aqueous media and esterification in organic solvents.
  • Their application is crucial for synthesizing valuable chemical compounds.

Purpose of the Study:

  • To review recent methodological advancements impacting lipase activity and enantioselectivity.
  • To highlight practical applications of lipases in producing chiral intermediates.
  • To provide insights into optimizing lipase-catalyzed reactions.

Main Methods:

  • Literature review of recent advancements in lipase methodology.
  • Analysis of factors influencing lipase activity and enantioselectivity.

Related Experiment Videos

  • Compilation of practical examples in chiral synthesis.

    • Main Results:

    • Identification of key practical factors enhancing lipase performance.
    • Demonstration of lipase utility in synthesizing enantiomerically pure intermediates.
    • Overview of successful lipase-mediated synthetic strategies.

    Conclusions:

    • Methodological progress has significantly improved lipase applications in organic synthesis.
    • Lipases offer efficient and selective routes to chiral compounds.
    • Further optimization of practical conditions will expand their synthetic utility.