Related Experiment Videos
Development of a genetic selection for catalytic antibodies
Jeff Gildersleeve1, Jeff Janes, Helle Ulrich
1Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Bioorganic & Medicinal Chemistry Letters
|June 1, 2002
Abstract:
The design and evaluation of a new genetic selection system for evolving catalytic antibodies with aldolase activity are described. Through a series of model selections, we have identified selection conditions where expression of a catalytically active antibody confers a growth advantage to Escherichia coli. In addition, we provide evidence that the growth advantage is a direct result of catalytic activity.