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Rapid detection of Escherichia coli O157:H7 with multiplex real-time PCR assays

Narayanan Jothikumar1, Mansel W Griffiths

  • 1Department of Food Science and Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Applied and Environmental Microbiology
|June 1, 2002
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...