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Expression of Exogenous E-cadherin Regulates Anchorage-independent Growth in Human Lung Adenocarcinoma Cells
Chuan-Xin Huang1, She Chen, Miao Ying
1Department of Biochemistry, Medical Center of Fudan University, Shanghai 200032, China. zhshen@shmu.edu.cn
Abstract:
To explore the suppressive effects of E-cadherin on aggregation and anchorage-independent growth of human lung adenocarcinoma cells, a mammalian expression vector containing full E-cadherin cDNA was constructed and transfected into a metastatic human lung adenocarcinoma cell line A549. RT-PCR and Western blot were used to analyse expression levels of E-cadherin. Cells aggregation and anchorage-independent growth of the tumor cells before and after gene transfection were assessed respectively. The results indicated that stable transfectants showed markedly increased levels of expressed E-cadherin compared with the corresponding sham transfectants. The transfections showed more intense cellular aggregation and anchorage-independent growth.
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