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Human ovarian tissue cryopreservation: indications and feasibility
Catherine Poirot1, Marie-Cécile Vacher-Lavenu, Pierre Helardot
1Laboratoire de Biologie de la Reproduction, Groupe Hospitalier Pitié-Salpêtrière, Université Paris VI, 75013 Paris, France. catherin.poirot@psl.ap-hop-paris.fr
Human Reproduction (Oxford, England)
|June 4, 2002
Summary
Ovarian tissue cryopreservation is feasible for women at risk of infertility due to gonadotoxic treatments. This method offers fertility preservation options for young women and girls facing sterility.
Area of Science:
- Reproductive Medicine
- Cryobiology
- Oncology Supportive Care
Background:
- Gonadotoxic treatments pose a risk of infertility in women.
- Ovarian tissue cryopreservation offers a potential solution for future fertility.
Purpose of the Study:
- To evaluate the feasibility of long-term ovarian tissue cryopreservation.
- To assess cryopreservation viability in women at risk of treatment-induced sterility.
Main Methods:
- Cryopreservation of ovarian tissue using slow cooling with DMSO and sucrose.
- Histological evaluation of ovarian cortex to determine follicle concentration.
- Analysis of 51 women at risk of sterility, aged 2.7-34 years.
Main Results:
- Ovarian tissue cryopreservation was successful in 31 patients.
- Follicle concentration varied significantly by age, with higher counts in younger individuals.
- An average of 26 ovarian fragments were cryopreserved per patient.
Conclusions:
- Ovarian tissue cryopreservation is a viable option for fertility preservation.
- This procedure should be systematically offered to young women and girls at risk of sterility.
- Enables future fertility through autografts or in-vitro follicle growth.