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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
Published on: May 10, 2016
Experimental study on apoptosis of HL-60 cell induced by arsenic trioxide
1Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Bejing (10091).
Objective:
To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia.
Methods:
The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed.
Results:
After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later.
Conclusion:
Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.
