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Published on: November 1, 2011
[Newly isolated mink parvovirus strain]
Abstract:
A new mink enteritis virus (MEV) strain, called Cherepanovo, was isolated in the Novosibirsk region (West Siberia). The level of this strain's accumulation in cell culture is lower than that of commercial MEV strains Rodniki or Beregovoi. Identification of Cherepanovo strain by hemagglutination inhibition test, protein electrophoresis in denaturing polyacrylamide gel, and PCR showed its similarity to the previously described MEV strains. Comparative analysis of this strain's 5' and 3' nucleotide sequences of VP2 gene showed its more than 98% homology with other feline parvoviruses. Nucleotide residues 2920, 2971, and 4245 in the Cherepanovo strain VP2 gene differed from those of all previously described MEV strains. It is noteworthy that transition at the position 4245 led to Phe-->Cys replacement in amino acid residue.
Insights
A novel mink enteritis virus (MEV) strain, Cherepanovo, was identified in West Siberia. Genetic analysis revealed its similarity to other MEV strains but with unique VP2 gene variations.
Area of Science:
- Virology
- Molecular Biology
- Animal Health
Background:
- Mink enteritis virus (MEV) causes significant morbidity and mortality in mink populations.
- Surveillance and characterization of novel viral strains are crucial for understanding disease dynamics and developing effective control strategies.
Purpose of the Study:
- To isolate and characterize a newly identified strain of mink enteritis virus (MEV) from West Siberia.
- To compare the genetic makeup of the novel strain with existing MEV strains and related parvoviruses.
Main Methods:
- Isolation of the virus in cell culture.
- Identification using hemagglutination inhibition tests, SDS-PAGE, and PCR.
- Comparative nucleotide sequence analysis of the VP2 gene (5' and 3' regions).
Main Results:
- A new MEV strain, designated Cherepanovo, was isolated in the Novosibirsk region.
- The Cherepanovo strain showed lower accumulation levels in cell culture compared to commercial strains.
- Genetic analysis confirmed high homology (>98%) with other feline parvoviruses, but identified unique nucleotide variations (2920, 2971, 4245) in the VP2 gene, including a Phe-->Cys amino acid substitution at position 4245.
Conclusions:
- The Cherepanovo strain represents a distinct genetic variant of MEV.
- The identified genetic differences may influence viral properties, warranting further investigation into its pathogenicity and epidemiological significance.
