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[Newly isolated mink parvovirus strain].
Voprosy Virusologii
|June 6, 2002
Summary
A novel mink enteritis virus (MEV) strain, Cherepanovo, was identified in West Siberia. Genetic analysis revealed its similarity to other MEV strains but with unique VP2 gene variations.
Area of Science:
- Virology
- Molecular Biology
- Animal Health
Background:
- Mink enteritis virus (MEV) causes significant morbidity and mortality in mink populations.
- Surveillance and characterization of novel viral strains are crucial for understanding disease dynamics and developing effective control strategies.
Purpose of the Study:
- To isolate and characterize a newly identified strain of mink enteritis virus (MEV) from West Siberia.
- To compare the genetic makeup of the novel strain with existing MEV strains and related parvoviruses.
Main Methods:
- Isolation of the virus in cell culture.
- Identification using hemagglutination inhibition tests, SDS-PAGE, and PCR.
- Comparative nucleotide sequence analysis of the VP2 gene (5' and 3' regions).
Main Results:
- A new MEV strain, designated Cherepanovo, was isolated in the Novosibirsk region.
- The Cherepanovo strain showed lower accumulation levels in cell culture compared to commercial strains.
- Genetic analysis confirmed high homology (>98%) with other feline parvoviruses, but identified unique nucleotide variations (2920, 2971, 4245) in the VP2 gene, including a Phe-->Cys amino acid substitution at position 4245.
Conclusions:
- The Cherepanovo strain represents a distinct genetic variant of MEV.
- The identified genetic differences may influence viral properties, warranting further investigation into its pathogenicity and epidemiological significance.