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Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

Quantification of digesta flow into the caeca.

J H Son¹, D Ragland, O Adeola

¹Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907-1151, USA. shinshu@chollian.net

British Poultry Science

|June 6, 2002

Summary

A new caecal intubation technique in chickens reveals that the ceca are crucial for water balance. Feeding significantly impacts digesta excretion, highlighting the cecum

Area of Science:

Animal Science
Avian Physiology
Digestive Physiology

Background:

The function of the chicken cecum in digesta and water balance is not fully understood.
Previous methods for studying cecal function in vivo have limitations.

Purpose of the Study:

To develop and validate a caecal intubation technique for assessing digesta flow in chickens.
To quantify the contribution of the cecum to dry matter and water balance in fed and feed-deprived chickens.

Main Methods:

A novel caecal intubation technique was employed in chickens.
Measurements included dry matter intake, water intake, and cecal excretion in both fed and feed-deprived states.
Control and caecostomized birds were utilized for comparison.

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Main Results:

Dry matter intake did not differ significantly between control and caecostomized chickens.
Water intake increased significantly with caecostomy and with feeding.
Feeding significantly increased cecal dry matter excretion, but not cecal water excretion.
The ratio of cecal to total excretion for dry matter and water decreased with feeding.

Conclusions:

The cecum plays a significant role in the water balance of chickens.
Dry matter enters the cecum, representing 18-25% of total dry matter excretion.
Water enters the cecum, accounting for 17-26% of total water excretion.