Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular cholesterol flux studies: methodological considerations.

G H Rothblat1, M de la Llera-Moya, E Favari

  • 1Department of Pediatrics, Division of Gastroenterology and Nutrition, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Abramson Research Center, Philadelphia, PA 19104-4318, USA. rothblat@email.chop.edu

Atherosclerosis
|June 6, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lomitapide affects HDL composition and function.

Atherosclerosis·2016
Same author

The natural compound berberine positively affects macrophage functions involved in atherogenesis.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2014
Same author

Flow-mediated dilation, carotid wall thickness and HDL function in subjects with hyperalphalipoproteinemia.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2014
Same author

Cellular cholesterol efflux and cholesterol loading capacity of serum: effects of LDL-apheresis.

Journal of lipid research·2012
Same author

Human C-reactive protein promotes oxidized low density lipoprotein uptake and matrix metalloproteinase-9 release in Wistar rats.

Journal of lipid research·2008
Same author

Scavenger receptor class B type I affects cholesterol homeostasis by magnifying cholesterol flux between cells and HDL.

Journal of lipid research·2001
Same journal

Optimized flow cytometry assay for functional characterization of variants of uncertain significance in familial hypercholesterolemia.

Atherosclerosis·2026
Same journal

Causal insights of modifiable cardiovascular risk factors for dementia risk - potential for efficient prevention and improved brain health.

Atherosclerosis·2026
Same journal

Serial OCT-based coronary physiology and plaque composition in vessels with nonobstructive coronary lesions following intensive lipid-lowering therapy: YELLOW III sub-study.

Atherosclerosis·2026
Same journal

Earliest age to detect lifetime cardiometabolic health stratification in children.

Atherosclerosis·2026
Same journal

Sympathetic neurons exacerbate atherosclerosis by modulating macrophage function via the NPY/Y1R axis.

Atherosclerosis·2026
Same journal

Optimizing lipoprotein(a) testing for immediate clinical impact in primary prevention.

Atherosclerosis·2026
See all related articles

Reverse cholesterol transport (RCT) removes excess cholesterol from cells. This review details methods for measuring cholesterol efflux and influx, crucial for understanding RCT and related diseases.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Reverse cholesterol transport (RCT) is vital for preventing cholesterol buildup in peripheral tissues.
  • RCT involves cholesterol release from cells to acceptors like high-density lipoprotein (HDL) for liver excretion.
  • Cellular cholesterol flux encompasses efflux, influx, and net flux, each requiring specific measurement techniques.

Purpose of the Study:

  • To review methodological considerations for designing and analyzing cellular cholesterol flux experiments.
  • To highlight the importance of accurate flux quantitation in understanding RCT.
  • To discuss recent advances in RCT research, including the roles of SR-B1 and ABCA1.

Main Methods:

  • Discussion of experimental protocols for measuring cholesterol efflux.

Related Experiment Videos

  • Evaluation of methods for quantifying cholesterol influx.
  • Considerations for determining net cholesterol flux.

    • Main Results:

    • Different experimental approaches are necessary for quantifying efflux, influx, and net flux.
    • Accurate measurement of cholesterol flux is essential for studying RCT.
    • Recent discoveries of SR-B1 and ABCA1 have renewed interest in cellular cholesterol flux studies.

    Conclusions:

    • Careful design and analysis of experiments are critical for accurate cellular cholesterol flux measurements.
    • Understanding cholesterol flux is key to elucidating the mechanisms of RCT.
    • This review provides a framework for researchers investigating cellular cholesterol metabolism and RCT.