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[Identification of genes associated with human osteosarcoma metastasis suppression using suppression subtractive
Jianning Yin1, Qingyu Fan, Xinbao Hao
1Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military University, Xi'an, Shanxi, 710038 P. R. China. yinjning@pub.xaonline.com
Objective:
To identify genes associated with metastasis suppression and to investigate the molecular mechanism of osteosarcoma metastasis.
Methods:
The subtracted cDNA library of low metastatic human osteosarcoma cell line SOSP-9607 was constructed using suppression subtractive hybridization. Partial clones were sequenced. The acquired sequence data were aligned against the GenBank nucleotide database using Blastn to search for sequence matches. The interested clone was used to perform Northern blot and reverse transcriptase-PCR (RT-PCR) analysis on mRNA isolated from low metastatic cell line SOSP-9607 and OS-9901, high metastatic cell line SOSP-M and three pulmonic metastatic nodules of nude mice.
Results:
A cDNA clone from low metastatic cell line SOSP-9607 subtracted cDNA library was identified as telomeric repeat binding factor 2 (TERF2) by sequence analysis and Blastn search. Northern blot and RT-PCR analysis demonstrated that TERF2 expressed highly in low metastatic cell line SOSP-9607 and OS-9901, but not in high metastatic cell line SOSP-M and three pulmonic metastatic nodules.
Conclusion:
TERF2 may be important for suppressing metastasis of osteosarcoma.
Insights
Telomeric repeat binding factor 2 (TERF2) may suppress osteosarcoma metastasis. This gene was highly expressed in low metastatic osteosarcoma cells but absent in highly metastatic cells and lung nodules.
Area of Science:
- Molecular biology
- Cancer research
- Genetics
Context:
- Osteosarcoma is a primary bone cancer with a high propensity for metastasis.
- Understanding the molecular mechanisms underlying osteosarcoma metastasis is crucial for developing effective treatments.
Purpose:
- To identify genes involved in osteosarcoma metastasis suppression.
- To investigate the role of identified genes in the molecular mechanism of osteosarcoma metastasis.
Summary:
- A subtracted cDNA library from a low-metastatic human osteosarcoma cell line (SOSP-9607) was used to identify metastasis-suppressing genes.
- Sequence analysis identified a clone as telomeric repeat binding factor 2 (TERF2).
- Northern blot and RT-PCR confirmed high TERF2 expression in low-metastatic cell lines and its absence in high-metastatic cells and metastatic nodules.
Impact:
- TERF2 may play a significant role in suppressing osteosarcoma metastasis.
- This finding could lead to new therapeutic strategies targeting TERF2 for osteosarcoma treatment.