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[Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy]
Xun Wang1, Youmei Xie, Cheng Zhang
1Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080 P. R. China. czym@gzsums.edu.cn
Objective:
To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.
Methods:
Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.
Results:
The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.
Conclusion:
Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.