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Updated: Sep 14, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
A note on the preparation of particulate material for an electron microscopic study
Abstract:
Modifications of two rapid and satisfactory procedures of purification of minute biological particulate material for electron microscopic observations were described. These modifications eliminate the alterations of the investigated particles and the possibility of their artificial disintegration. In addition, these procedures preserve the grouping of the particles as compared with the original suspensions. The dialysis is made directly on the grids coated with the supporting formvar membrane. In the second procedure the filtration of the soluble components of the material into the agar is combined with the transfer of the particles on the membrane covered grids.
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Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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