Horizontal distribution of three dehydrogenases in the cow snout epidermis

A technique is described for quantifying enzyme activities in the various layers of the epidermis of the cow snout. Values for glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and lactate dehydrogenase are reported. In general, the amount of each enzyme is largest in the basal layers and diminished towards the stratum corneum. The exception was G6PD whose highest peak of activity was in the region of the stratum spinosum.