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LC determination of enrofloxacin
Marinês J e Souza1, Celso F Bittencourt, Lisoni M Morsch
1Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, CEP 97, RS, Santa Maria, Brazil. msouza@ccs.ufsm.br
Journal of Pharmaceutical and Biomedical Analysis
|June 7, 2002
Summary
A new high-performance liquid chromatographic (HPLC) method accurately quantifies enrofloxacin in raw materials and injections. This validated method offers a sensitive and reliable approach for enrofloxacin analysis.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Enrofloxacin is a crucial fluoroquinolone antibiotic widely used in veterinary medicine.
- Accurate quantification of enrofloxacin is essential for quality control and therapeutic efficacy.
Purpose of the Study:
- To develop and validate a simple, rapid, and sensitive high-performance liquid chromatographic (HPLC) method for enrofloxacin assay.
- To determine the suitability of the method for analyzing enrofloxacin in raw material and injection formulations.
Main Methods:
- Reversed-phase HPLC using a C-18 column.
- Isocratic elution with a mobile phase of sodium acetate and acetonitrile.
- UV detection at 278 nm.
- Method validation including linearity, precision, accuracy, LOD, and LOQ determination.
Main Results:
- The developed HPLC method demonstrated good linearity over the range of 10.0 to 80.0 microg ml(-1).
- Comprehensive validation confirmed the method's reliability, accuracy, and sensitivity.
- The method is suitable for the quantitative assay of enrofloxacin in both raw material and injection forms.
Conclusions:
- A validated, sensitive, and rapid HPLC method for enrofloxacin analysis has been successfully established.
- This method provides a robust tool for quality control of enrofloxacin products.
- The assay is suitable for routine analysis in pharmaceutical settings.