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Related Experiment Videos

Automatic potentiometric flow titration procedure for ascorbic acid determination in pharmaceutical formulations.

Ana Paula S Paim1, Cristina M N V Almeida, Boaventura F Reis

  • 1Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Av. Centenário 303, P.O. Box 96, 13400-970 Piracicaba, São Paulo, Brazil.

Journal of Pharmaceutical and Biomedical Analysis
|June 7, 2002
PubMed
Summary

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A new flow method accurately determines ascorbic acid in pharmaceuticals using potentiometric titration. This robust technique offers reliable results comparable to manual methods with high precision.

Area of Science:

  • Analytical Chemistry
  • Electrochemistry
  • Pharmaceutical Analysis

Background:

  • Ascorbic acid (vitamin C) is a vital compound in pharmaceuticals.
  • Accurate determination of ascorbic acid is crucial for quality control in pharmaceutical formulations.
  • Existing manual titration methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and validate a flow-based potentiometric titration procedure for ascorbic acid determination.
  • To implement a microcomputer-controlled flow network for enhanced operational efficiency and robustness.
  • To assess the method's performance in pharmaceutical formulations.

Main Methods:

  • Utilized a flow-through ion selective electrode for iodide detection.
  • Employed potentiometric titration based on the reduction of iodate (IO3-) by ascorbic acid.

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  • Implemented multicommutation within a microcomputer-controlled flow network for automated operation.

    • Main Results:

    • The developed flow system accurately determined ascorbic acid in pharmaceutical formulations within a concentration range of 7.5 to 15.0 mmol L(-1).
    • Results showed no significant difference (95% confidence level) compared to a conventional manual titration procedure.
    • Achieved a relative standard deviation of 1.0% (n=6) and a reagent consumption of 21.4 mg IO3- per determination.

    Conclusions:

    • The described flow procedure provides a robust and accurate method for the determination of ascorbic acid in pharmaceutical formulations.
    • The microcomputer-controlled multicommutation system enhances ease of operation and reliability.
    • This automated flow method is a viable alternative to manual procedures for quality control of ascorbic acid in pharmaceuticals.