Vitrification of human spermatozoa without cryoprotectants
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Updated: Oct 1, 2026
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
F Nawroth1, V Isachenko, S Dessole
1Department of Obstetrics and Gynecology, University of Cologne, Kerpener Str. 34, 50931, Germany.
Abstract:
The aim of our study was to investigate the effects of vitrification (cooling rate approximately 10000(C/min) without cryoprotectants on swim-up prepared human spermatozoa in comparison to standard conventional freezing with cryoprotectants. Motility, morphology, rate of viability and acrosome reaction of spermatozoa were evaluated. The described method of cryopreservation of human spermatozoa by direct plunging into liquid nitrogen slush without cryoprotectants was effective and could be recommended for routine IVF.
