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Updated: Jun 24, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Hypochlorite and Cu(2 ) Induced Oxidation of High Density Lipoprotein
1Apolipoproteins Research Unit, Institute of Biochemistry and Molecular Biology, West China University of Medical Sciences, Chengdu 610041, China. fucarl@mail.sc.cninfo.net
Abstract:
In order to further study on the properties of oxidatively modified high density lipoproteins(Ox-HDL), the effects of hypochlorite and Cu(2 ) on HDL oxidation in vitro were investigated. The results showed that, as to LDL, HDL was also oxidized by hypochlorite and Cu(2 ), causing the formation of thiobarbituric acid substances (TBARS) and increase of negative charges on HDL particle surface. It was also found that during the oxidation of hypochlorite, both apoAI and apoAII were degraded into small fragments, the phosphatidylcholine(PC) hydrolyzed into lysophosphatidylcholine (LPC) and the protein components of HDL formed carbonyls.
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