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Cloning and Expression Analysis of a Laryngeal Carcinoma Related Gene, LCRG1
You-Jun Li1, Hai-Long Xie, Zhu-Chu Chen
1Cancer Research Institution of XiangYa Medical School of Central Southern Universituy, Changsha 410078, China. youjun_li@yahoo.com
Abstract:
By screening a human adult cDNA library using a cDNA fragment (AF10056) as a probe, which is significantly down-regulated in laryngeal carcinoma and represents a novel gene, a cDNA, LCRG1(laryngeal carcinoma related gene 1) was identified, which was significantly down-regulated in 12 of 30(40%) primary laryngeal carcinomas and in 6 of 11(54.5%) various cancer cell lines. This gene, localized on chromosome band 17q12--21.1 by alignment of it with STS markers, was composed of six exons and spaned about 60 kb of genomic DNA with a 3.4 kb mature transcript. The putative protein encoded by this gene was 288 amino acid with no significant homology with any known proteins in databases. LCRG1 was expressed in many tissues, as shown by MTN blot analysis. These data suggest that LCRG1 is related to the laryngeal carcinoma.
Insights
Researchers identified a novel gene, LCRG1 (laryngeal carcinoma related gene 1), which is significantly down-regulated in laryngeal tumors. This finding suggests LCRG1 plays a role in laryngeal cancer development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Laryngeal carcinoma is a significant global health concern.
- Identifying novel genes involved in cancer development is crucial for understanding disease mechanisms.
Purpose of the Study:
- To identify and characterize novel genes associated with laryngeal carcinoma.
- To investigate the expression levels of a newly identified gene, LCRG1, in laryngeal cancer.
Main Methods:
- Screening of a human adult cDNA library using a down-regulated cDNA fragment (AF10056) as a probe.
- Gene expression analysis (MTN blot) in primary laryngeal carcinomas and cancer cell lines.
- Chromosomal localization using STS markers.
Main Results:
- Identification of a novel cDNA, LCRG1 (laryngeal carcinoma related gene 1).
- LCRG1 was significantly down-regulated in 40% of primary laryngeal carcinomas and 54.5% of cancer cell lines.
- LCRG1 gene maps to chromosome band 17q12--21.1 and encodes a 288-amino acid protein with no significant homology to known proteins.
- LCRG1 exhibits expression in various tissues.
Conclusions:
- LCRG1 is a novel gene potentially involved in the development of laryngeal carcinoma.
- The down-regulation of LCRG1 in laryngeal tumors suggests a tumor-suppressive role.