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Chromatographic approach for determining the relative membrane permeability of drugs
Qing C Meng1, Jonas S Johansson, Roderic G Eckenhoff
1Center for Research in Anesthesia, Department of Anesthesia, 7 Dulles, University of Pennsylvania Health System, 3400 Spruce Street, Philadelphia, PA 19104, USA.
Abstract:
With the aid of the experimental dependence of the theoretical plate height (H) on the flow-rate (U), values of diffusion coefficients as the permeation rate, of the compounds in a polymeric stationary phase were calculated from solute mass transfer. This approach is proposed for modeling the relative diffusion rate of a drug within the membrane. After the successful separation of opioid compounds using a C(18) derivatized polystyrene-divinylbenzene polymer HPLC column, the slopes of H-U plots increase quantitatively in the order of meperidine