Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Current issues in cochlear gene transfer.

Anil K Lalwani1, Jussi Jero, Anand N Mhatre

  • 1Epstein Laboratories, Department of Otolaryngology, Head and Neck Surgery, University of California, San Francisco, CA 94143-0526, USA. lalwani@itsa.ucsf.edu

Audiology & Neuro-Otology
|June 8, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does Gamma Knife Stereotactic Radiosurgery Preserve Hearing on Short- and Long-Term Follow-Up?

The Laryngoscope·2026
Same author

Otological conditions and autism spectrum disorder in Medicaid beneficiaries aged 1 to 20 years: a cross-sectional study.

BMC pediatrics·2026
Same author

Multi-Task Assessment of Context-Specific Gait Changes to Virtual Reality-Based Visual Perturbations.

The Laryngoscope·2026
Same author

Reply: comment on "Superior semicircular abnormalities are more common in patients presenting to the emergency room with unexplained vertigo".

Clinical imaging·2026
Same author

A retrospective study on differences in neuroborreliosis symptoms, signs and findings between adults and children.

BMC infectious diseases·2026
Same author

Music Enjoyment and Emotional Response to Music in Cochlear Implant Users are Independent of Listening Modality.

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·2025
Same journal

Sudden Traumatic Noise Exposure Induces an altered wave I in the Auditory Brainstem Response in Human.

Audiology & neuro-otology·2026
Same journal

Test Equivalency of the Spanish and English AzBio Sentences Among Bilingual Normal Hearers.

Audiology & neuro-otology·2026
Same journal

Inner Ear Test Battery Aligned with Hydrops Magnetic Resonance Imaging to Differentiate Acute-Onset Sensorineural Hearing Loss.

Audiology & neuro-otology·2026
Same journal

Audiometric Outcomes and Longitudinal Trends of Sequential Cochlear Implantation in Adults with Bilateral Deafness: A 20-Year Single-Center Study.

Audiology & neuro-otology·2026
Same journal

Pediatric Robotic Cochlear Implantation: Hearing Outcomes.

Audiology & neuro-otology·2026
Same journal

A Prospective Study on the Diagnostic Accuracy of the Smartphone Weber Test in Unilateral Hearing Loss.

Audiology & neuro-otology·2026
See all related articles

Cochlear gene therapy offers a promising approach to treating deafness. Researchers evaluated various delivery methods and vectors, finding Gelfoam((R)) application to the round window membrane safe and effective for gene delivery.

Area of Science:

  • Otolaryngology
  • Gene Therapy
  • Molecular Biology

Background:

  • Cochlear gene therapy is a developing field for understanding and treating hearing loss.
  • Effective gene transfer to the cochlea requires careful selection of delivery routes and vectors.
  • Previous studies have explored various methods for introducing genetic material into the inner ear.

Purpose of the Study:

  • To evaluate different delivery methods for cochlear gene transfer.
  • To assess the efficacy and safety of various gene transfer vectors for inner ear applications.
  • To identify optimal strategies for future clinical translation of cochlear gene therapy.

Main Methods:

  • Delivery routes tested: osmotic minipump infusion, direct cochlear microinjection, and Gelfoam((R)) application to the round window membrane.

Related Experiment Videos

  • Gene transfer vectors investigated in vivo: cationic liposomes, adeno-associated virus (AAV), adenovirus, lentivirus, herpes simplex virus (HSV), and vaccinia virus.
  • Evaluation of vector efficacy, utility, and safety for intracochlear gene transfer.

    • Main Results:

    • Gelfoam((R)) application to the round window membrane was identified as an easy, safe, and atraumatic delivery technique.
    • Several viral vectors (AAV, adenovirus, lentivirus, HSV, vaccinia virus) and nonviral vectors (cationic liposomes) demonstrated successful in vivo gene transfer.
    • Viral vectors show experimental advantages, but nonviral vectors are preferred for future human gene therapy due to safety.

    Conclusions:

    • The Gelfoam((R)) method provides a practical approach for cochlear gene delivery.
    • A range of viral and nonviral vectors are suitable for experimental intracochlear gene transfer.
    • Future human cochlear gene therapy will likely prioritize nonviral vectors, addressing safety concerns regarding vector dissemination is crucial.