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Barotolerant E.coli Induced by High Hydrostatic Pressure.
Xiang Gao1, Jiong Li, Kang-Cheng Ruan
1Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. kcruan@sunm.shcnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|June 8, 2002
Summary
High hydrostatic pressure treatment enhanced Escherichia coli survival rates. Barotolerant strains showed increased survival and highly expressed proteins, including one with high identity to an outer membrane protein.
Area of Science:
- Microbiology
- Biochemistry
- Proteomics
Background:
- Escherichia coli (E. coli) is a common bacterium.
- High hydrostatic pressure (HHP) can be used to induce stress responses in bacteria.
- Understanding bacterial adaptation to environmental stress is crucial for various applications.
Purpose of the Study:
- To develop barotolerant strains of E. coli using HHP treatment.
- To investigate the proteomic changes associated with barotolerance in E. coli.
Main Methods:
- Escherichia coli strains (TG1, DH5alpha, HB101) were subjected to HHP treatment.
- Survival rates of wild-type and barotolerant strains were determined.
- Partial proteomes of wild-type and barotolerant strains were compared using two-dimensional electrophoresis.
Main Results:
- HHP treatment significantly increased the survival rates of E. coli strains TG1, DH5alpha, and HB101 by 2-3 orders of magnitude.
- Two-dimensional electrophoresis revealed three proteins were significantly overexpressed in barotolerant E. coli.
- One identified protein has an N-terminal sequence (V(L)EAGEFFMRA) and molecular weight (21 kD) consistent with an E. coli outer membrane protein.
Conclusions:
- HHP treatment is an effective method for generating barotolerant E. coli strains.
- The observed proteomic alterations, particularly the overexpression of specific proteins, contribute to enhanced barotolerance in E. coli.
- The identified outer membrane protein may play a role in E. coli's adaptation to high-pressure environments.