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[The effect of inactivation of co-stimulated particles B7.1 (CD80), B7.2 (CD86) on T lymphocyte activity]

Ryszard Rutkowski1, Tadeusz Moniuszko, Anna Stasiak-Barmuta

  • 1Klinika Alergologii i Chorób Wewnetrznych Akademii Medycznej w Białymstoku.

Polski Merkuriusz Lekarski : Organ Polskiego Towarzystwa Lekarskiego
|June 11, 2002
PubMed

We have studied the effect of CD80 (B7.1) and CD86 (B7.2) costimulatory molecules inactivation on IL-4 and IFN-gamma production by T lymphocytes. T cells were received from nonatopic asthmatic and healthy subjects. We have added anti-CD80 or anti-CD86 monoclonal antibodies to the monocyte/lymphocyte (1:5) cultures. In nonatopic asthma patients compare to control group inactivation of CD80 significantly reduced IFN-gamma production by T lymphocytes (2172/2368 pg/ml; p < 0.02). Blockade of CD86 decreased IFN-gamma production non-significantly (2858/3317 pg/ml; p < 0.3). In both studied groups anti-CD80 antibodies did not diminish T lymphocyte production of IL-4 (control: 630/725 pg/ml; nonatopic asthma: 718/742 pg/ml). Anti-CD86 antibodies reduced the IL-4 concentration in culture supernatants (630/523 pg/ml; 718/659 pg/ml), but the changes were nonsignificant.

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